TABLE 1.
Plasmids used in this study
| Plasmid | Descriptiona | Replicon | Selective markerb | Source or reference |
|---|---|---|---|---|
| Plasmids used in CHL resistance assay | ||||
| pMG2/pMG3 | Promoter reporter vector | pMB1 | AMP (CHL) | 7, 19 |
| pSU18 | Cloning vector | p15A | CHL | 6 |
| pRM50 | pSU18 BamHI Ω(PM2 1583-77) | p15A | CHL | This study |
| pRM52 | pSU18 BamHI Ω(PM2 850-3719) | p15A | CHL | This study |
| pRM950 | pMG2 SmaI Ω(PM2 77-1583) | pMB1 | AMP (CHL) | This study |
| pRM950.2 | pMG2 SmaI Ω(PM2 1583-77) | pMB1 | AMP (CHL) | This study |
| pRM952 | pMG2 SmaI Ω(PM2 850-3719) | pMB1 | AMP (CHL) | This study |
| pRM952.2 | pMG2 SmaI Ω(PM2 3719-850) | pMB1 | AMP (CHL) | This study |
| pRM958 | pMG2 Δ(XbaI-SacI) Ω(PM2 1129-1358) | pMB1 | AMP (CHL) | This study |
| pRM975 | pMG2 Δ(XbaI-SacI) Ω(PM2 5272-5405) | pMB1 | AMP (CHL) | This study |
| pRM984 | pMG2 Δ(XbaI-BamHI) Ω(PM2 4782-5818) | pMB1 | AMP (CHL) | This study |
| pRM1054 | pMG3 Ω(PM2 1583-551, 542-77) | pMB1 | AMP (CHL) | This study |
| pRM1059 | pMG3 Δ(SacI-XbaI) Ω(PM2 1358-1129) | pMB1 | AMP (CHL) | This study |
| pRM992 | pMG2 Δ(XbaI-SacI) Ω(PM2 77-1583), P1193 mutated | pMB1 | AMP (CHL) | This study |
| pRM993 | pMG2 Δ(XbaI-SacI) Ω(PM2 77-1583), P1253 mutated | pMB1 | AMP (CHL) | This study |
| pRM994 | pMG2 Δ(XbaI-SacI) Ω(PM2 3719-850), P1207 mutated | pMB1 | AMP (CHL) | This study |
| pRM1092 | pMG3 Δ(SacI-XbaI) Ω(PM2 1583-77), P1193 mutated | pMB1 | AMP (CHL) | This study |
| pRM1094 | pMG3 Δ(SacI-XbaI) Ω(PM2 850-3719), P1207 mutated | pMB1 | AMP (CHL) | This study |
| Plasmids used to study viral transcriptional activators | ||||
| pALHI | Expression vector | p15A | KAN | This study |
| pRM513 | pALHI Δ(EcoRI-BamHI) Ω(PM2 3702-3910) | p15A | KAN | This study |
| pRM521 | pALHI Δ(EcoRI-BamHI) Ω(PM2 561-77) | p15A | KAN | This study |
| pRM531 | pALHI Δ(EcoRI-BamHI) Ω(PM2 4196-4643) | p15A | KAN | This study |
| pRM533 | pALHI Δ(EcoRI-BamHI) Ω(PM2 3892-4212) | p15A | KAN | This study |
| pRM590 | pALHI Δ(EcoRI-BamHI) Ω(PM2 3702-4212) | p15A | KAN | This study |
| pRM591 | pALHI Δ(EcoRI-BamHI) Ω(PM2 3702-4643) | p15A | KAN | This study |
| pRM595 | pALHI Δ(EcoRI-BamHI) Ω(PM2 1145-77) | p15A | KAN | This study |
| pRM596 | pALHI Δ(EcoRI-BamHI) Ω(PM2 1346-1822) | p15A | KAN | This study |
| pSN100 | pALHI Ω(PM2 1145-551, 100-77) | p15A | KAN | This study |
| pSN102 | pALHI Ω(PM2 1346-1397, 1564-1822) | p15A | KAN | This study |
| pSN104 | pALHI Ω(PM2 1346-1600, 1796-1822) | p15A | KAN | This study |
| pSN106 | pALHI Ω(PM2 1145-1087, 852-551, 100-77) | p15A | KAN | This study |
| pSN108 | pALHI Ω(PM2 1145-847, 553-551, 100-77) | p15A | KAN | This study |
a
Nucleotide coordinates of bacteriophage PM2 genome as previously described (33). Ω, insertion; Δ, deletion.
b
The selective marker indicated in parentheses is only expressed when a promoter sequence is inserted in front of it.