. 2003 Jun;185(11):3361–3372. doi: 10.1128/JB.185.11.3361-3372.2003

TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Description	Relevant phenotype^b	Source or reference
Strains
E. coli
HB101	F⁻ Δ(gpt-proA)62leuB6 supE44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Str^r) xyl-5 mtl-1 recA13		44
XL1-Blue	F′::Tn10 proA⁺B⁺lacI^q Δ(lacZ)M15/recA1 endA1 gyrA96 (Nal^r) thi hsdR17 (r_K⁻ m_K⁺) supE44 relA1 lac		Stratagene
RP4182	dam dcm		A. Bhagwat
R. capsulatus
MT1131^a	crtD121 Rif^r	Wild type, Nadi⁺ Ps⁺	47
Y262		GTA overproducer	55
MD1	Δ(ccdA::spe)	Nadi⁻ Ps⁻	13
MD1-Rev1A	Ps⁺ revertant of MD1 obtained on MYPE	Res⁺ Ps^slow on Med A, Res⁻ Ps⁺ on MPYE	This work
MD20	Δ(dsbA::kan)	Res⁺ Ps⁺ on Med A	This work
		Res⁻ Ps⁺ on MPYE
MD21	Δ(ccdA::spe dsbA::kan)	Res⁺ Ps^slow on Med A, Res⁻ Ps⁺ on MPYE	This work
MD22	Δ(dsbB::kan) obtained on Med A under respiratory growth conditions	Res⁺ Ps⁺ on Med A, Res⁺ Ps⁺ on MPYE	This work
MD23	Δ(dsbB::kan) obtained on Med A under photosynthetic growth conditions	Res⁺ Ps⁺ on Med A, Res⁻ Ps⁺ on MPYE	This work
MD24	Δ(ccdA::spe dsbB::kan) obtained on Med A under respiratory growth conditions	Res⁺ Ps^slow on Med A, Res⁺ Ps^slow on MPYE	This work
ST1, ST6, and ST7	MD1 revertants selected on Med A under photosynthetic growth conditions	Res⁺ Ps⁺ on Med A	This work
ST11, ST12, ST14, and ST18	MD1 revertants selected on MPYE under photosynthetic growth conditions	Res⁺ Ps⁺ on Med A	This work
MD1R1.13C	Ps⁺ revertant selected from MD1/pDsbA^WT and subsequently cured of its plasmid	Spe^r Res⁺	This work
Plasmids
pRK2013		Kan^r, helper	15
pRK404		Tet^r	15
pRK415		Tet^r	27
pCHB500		Tet^r	5
pHPΩ45-Km		Kan^r	40
pMA117		Kan^r	10
pBluescript	pBluescript II KS(+)	Amp^r	Stratagene
pTC4-1	6.2-kb noncontiguous chromosomal DNA fragments containing R. capsulatus dsbA in pRK415	Tet^r	This work
pTC4-1A	4.5-kb EcoRI fragment of pTC4-1 in EcoRI site of pRK415	Tet^r	This work
pTC4-1B	pTC4-1A without the 4.0-kb BamHI fragment	Tet^r	This work
pTC4-1C4	2.5-kb PstI fragment of pTC4-1 in PstI site of pRK415	Tet^r	This work
pTC4-1D	pTC4-1 without the 2.5-kb PstI fragment	Tet^r	This work
pTC4-1E	pTC4-1C4 without the 1,045-bp BamHI-BglII fragment	Tet^r	This work
pTC4-1G	2.5-kb PstI fragment of pTC4-1C4 in the PstI site of pBluescript	Amp^r	This work
pTC4-1H	521-bp BglII-SfiI fragment of pTC4-1G replaced with the 2.2-kb kanamycin resistance cassette	Amp^r Kan^r	This work
pTC4-1K	4.2-kb insert of pTC4-1H in XbaI and KpnI sites of pRK415	Tet^r Kan^r	This work
pBS-dsbA^WT	700-bp PCR product containing wild-type dsbA cloned into the SmaI site of pBluescript	Amp^r	This work
pDsbA^WT	700-bp XbaI and KpnI fragment of pBS-dsbA^WT cloned into the corresponding sites of pCHB500	Tet^r	This work
pBS-dsbB^WT	1.4-kb PCR product containing wild-type dsbB cloned into the XbaI and KpnI site of pBSII	Amp^r	This work
pDsbB^WT	XbaI and KpnI fragment of pBS-dsbB^WT cloned into the respective sites of pRK415	Tet^r	This work
pDA2	The Kan^r cartridge of pMA117 inserted in the unique PstI site of pBS-dsbB^WT; Kan^r and dsbB transcribed in opposite directions	Amp^r	This work
pDA4	XbaI and KpnI fragment of pDA2 cloned into sites of pRK4151	Tet^r	This work

R. capsulatus MT1131 is referred to as the wild type because it is the wild type with respect to its Cyt c profile and growth properties. All R. capsulatus strains, except Y262, are derivatives of MT1131, which was originally isolated as a green derivative of R. capsulatus SB1003 (47).

Ps^slow, slow growth under photosynthetic conditions.