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. 2003 Jun;185(11):3259–3269. doi: 10.1128/JB.185.11.3259-3269.2003

FIG. 7.

FIG. 7.

Capacity of VirB9 and VirB10 to form high-molecular-weight complexes in the presence or absence of VirB1 to VirB4. Samples from AS-induced strains were added to loading buffer with DTT (lanes +), added to loading buffer without DTT (lanes −), or cross-linked with BS3 and added to loading buffer with DTT (lanes x). Extracts from equivalent numbers of cells were then resolved by SDS-10% PAGE, blotted, and probed with anti-VirB10 (A) or anti-VirB9 (B) antibodies. Gel 1, A348; gel 2, A136(pAB123, pZL48-D5, pZL3) (virB6Δ185aa-10, virB1 to virB4); gel 3, A136(pAB123, pZL48-D5) (virB6Δ185aa-10).