FIG. 3.

Typical colonization after 8 h of appliance wear. Staining as done for Fig. 2. Panels A, B, and D are 8-h plaque samples that correspond directly (i.e., are from the same volunteer in the same experiment) to the 4-h samples shown in Fig. 2. Insets: electronic zoom of center region. (A) Colonial nature of plaque accumulation is apparent. Two anti-type-1-reactive cells are visible (arrowheads). Inset shows heterogeneity of anti-RPS reactivity within colonies and a single anti-type-1 reactive cell (arrowhead). (B) Long, weakly fluorescent rods and large, highly fluorescent cocci (center) are visible. No anti-type-1-reactive cells are visible. Inset confirms heterogeneity of anti-RPS reactivity within colonies. (C) Colonial nature of accumulation and heterogeneity of anti-RPS-reactive cells (purple) as well as of anti-DL1-reactive cells (green) within single colonies is apparent. Inset confirms heterogeneity of antibody reactivity within colonies; three groups of cells occur (anti-DL1 reactive, anti-RPS reactive, and antibody unreactive). (D) Colony composed of anti-RPS-reactive cells, anti-DL1-reactive cells, and antibody-unreactive cells is seen in lower right. Inset shows mixed colony of anti-RPS-reactive cells and Syto 59-stained cells; anti-DL1-reactive cells (green) are in close proximity to anti-RPS-reactive cells as well as in more solitary positions.