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. 1973 Nov;15(3):365–374.

Membrane-associated immunoglobulins of human lymphocytes in immunologic disorders

Isabelle Nicod, J P Girard, A Cruchaud
PMCID: PMC1553910  PMID: 4587505

Abstract

Membrane-associated immunoglobulins of peripheral blood lymphocytes were studied by indirect immunofluorescence for γ, α, μ, κ and λ chains in healthy subjects and patients with immunologic disease.

In healthy subjects, heavy chains were found on 30·7% of lymphocytes (γ 15·3%, α 7·2% and μ 8·2%) and light chains on 32·8% of cells (κ 20·4% and λ 12·4%). Patients with humoral immune deficiencies had fewer immunoglobulin-bearing cells; sarcoidosis or thymectomy patients had normal or decreased immunoglobulin-bearing lymphocytes; cells with light chains were fewer than those with heavy chains on their lymphocytes. In some cases, normal levels of serum immunoglobulins were found in the absence of the corresponding immunoglobulin-bearing cells, and in others normal immunoglobulin-bearing lymphocytes were present in the absence of the corresponding serum immunoglobulins.

These data suggest that (1) immunoglobulin-bearing lymphocytes in blood do not reflect the condition of immunoglobulin-synthesizing cells in peripheral lymphoid tissues, and (2) in certain immunologic disorders, either some B-lymphocytes do not synthesize immunoglobulins, or immunoglobulins are in such a situation that the whole molecule or part of the molecule is not visualized by current methods.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aisenberg A. C., Bloch K. J. Immunoglobulins on the surface of neoplastic lymphocytes. N Engl J Med. 1972 Aug 10;287(6):272–276. doi: 10.1056/NEJM197208102870603. [DOI] [PubMed] [Google Scholar]
  2. Bankhurst A. D., Warner N. L., Sprent J. Surface immunoglobulins on thymus and thymus-derived lymphoid cells. J Exp Med. 1971 Oct 1;134(4):1005–1015. doi: 10.1084/jem.134.4.1005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coombs R. R., Feinstein A., Wilson A. B. Immunoglobulin determinants on the surface of human lymphocytes. Lancet. 1969 Nov 29;2(7631):1157–1160. doi: 10.1016/s0140-6736(69)92484-2. [DOI] [PubMed] [Google Scholar]
  4. Cooper M. D., Lawton A. R., Bockman D. E. Agammaglobulinaemia with B lymphocytes. Specific defect of plasma-cell differentiation. Lancet. 1971 Oct 9;2(7728):791–794. doi: 10.1016/s0140-6736(71)92742-5. [DOI] [PubMed] [Google Scholar]
  5. Cooper M. D., Lawton A. R., Bockman D. E. Agammaglobulinaemia with B lymphocytes. Specific defect of plasma-cell differentiation. Lancet. 1971 Oct 9;2(7728):791–794. doi: 10.1016/s0140-6736(71)92742-5. [DOI] [PubMed] [Google Scholar]
  6. Cooper M. D., Lawton A. R. Circulating B-cells in patients with immunodeficiency. Am J Pathol. 1972 Dec;69(3):513–528. [PMC free article] [PubMed] [Google Scholar]
  7. Froland S. S., Natvig J. B. Surface-bound immunoglobulin on lymphocytes from normal and immunodeficient humans. Scand J Immunol. 1972;1(1):1–12. doi: 10.1111/j.1365-3083.1972.tb03730.x. [DOI] [PubMed] [Google Scholar]
  8. Fröland S., Natvig J. B., Berdal P. Surface-bound immunoglobulin as a marker of B lymphocytes in man. Nat New Biol. 1971 Dec 22;234(51):251–252. doi: 10.1038/newbio234251a0. [DOI] [PubMed] [Google Scholar]
  9. Girard J. P., Poupon M. F., Press P. Culture of peripheral blood lymphocytes from sarcoidosis; response to mitogenic factor. Int Arch Allergy Appl Immunol. 1971;41(4):604–619. doi: 10.1159/000230553. [DOI] [PubMed] [Google Scholar]
  10. Greaves M. F. Biological effects of anti-immunoglobulins: evidence for immunoglobulin receptors on 'T' and 'B' lymphocytes. Transplant Rev. 1970;5:45–75. doi: 10.1111/j.1600-065x.1970.tb00356.x. [DOI] [PubMed] [Google Scholar]
  11. Greaves M. F., Torrigiani G., Roitt I. M. Blocking of the lymphocyte receptor site for cell mediated hypersensitivity and transplantation reactions by anti-light chain sera. Nature. 1969 May 31;222(5196):885–886. doi: 10.1038/222885a0. [DOI] [PubMed] [Google Scholar]
  12. Grey H. M., Rabellino E., Pirofsky B. Immunoglobulins on the surface of lymphocytes. IV. Distribution in hypogammaglobulinemia, cellular immune deficiency, and chronic lymphatic leukemia. J Clin Invest. 1971 Nov;50(11):2368–2375. doi: 10.1172/JCI106735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lesley J. F., Kettman J. R., Dutton R. W. Immunoglobulins on the surface of thymus-derived cells engaged in the initiation of a humoral immune response. J Exp Med. 1971 Sep 1;134(3 Pt 1):618–629. doi: 10.1084/jem.134.3.618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Loh H. S., Wilson C. W. Iron and vitamin C. Lancet. 1971 Oct 2;2(7727):768–769. doi: 10.1016/s0140-6736(71)92135-0. [DOI] [PubMed] [Google Scholar]
  16. MONTREUIL J., CHOSSON A., HAVEZ R., MULLET S. [Isolation of beta2A-globulins from human milk]. C R Seances Soc Biol Fil. 1960;154:732–736. [PubMed] [Google Scholar]
  17. Mason S., Warner N. L. The immunoglobulin nature of the antigen recognition site on cells mediating transplantation immunity and delayed hypersentivity. J Immunol. 1970 Mar;104(3):762–765. [PubMed] [Google Scholar]
  18. Nossal G. J., Warner N. L., Lewis H., Sprent J. Quantitative features of a sandwich radioimmunolabeling technique for lymphocyte surface receptors. J Exp Med. 1972 Feb 1;135(2):405–428. doi: 10.1084/jem.135.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Papamichail M., Brown J. C., Holborow E. J. Immunoglobulins on the surface of human lymphocytes. Lancet. 1971 Oct 16;2(7729):850–852. doi: 10.1016/s0140-6736(71)90224-8. [DOI] [PubMed] [Google Scholar]
  20. Piessens W. F., Schur P. H., Moloney W. C., Churchill W. H. Lymphocyte surface immunoglobulins. Distribution and frequency in lymphoproliferative diseases. N Engl J Med. 1973 Jan 25;288(4):176–180. doi: 10.1056/NEJM197301252880403. [DOI] [PubMed] [Google Scholar]
  21. Preud'homme J. L., Seligmann M. Primary immunodeficiency with increased numbers of circulating B lymphocytes contrasting with hypogammaglobulinaemia. Lancet. 1972 Feb 19;1(7747):442–442. doi: 10.1016/s0140-6736(72)90898-7. [DOI] [PubMed] [Google Scholar]
  22. Uhr J. W. Passive sensitization of lymphocytes and macrophages by antigen-antibody complexes. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1599–1606. doi: 10.1073/pnas.54.6.1599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Van Boxel J. A., Paul W. E., Terry W. D., Green I. Communications. IgD-bearing human lymphocytes. J Immunol. 1972 Sep;109(3):648–651. [PubMed] [Google Scholar]
  24. Wood B. T., Thompson S. H., Goldstein G. Fluorescent antibody staining. 3. Preparation of fluorescein-isothiocyanate-labeled antibodies. J Immunol. 1965 Aug;95(2):225–229. [PubMed] [Google Scholar]

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