TABLE 1.
Primers used in this study
| Primera | Sequence (5′ to 3′)b |
|---|---|
| ChiA-F | TGCTCTAGAGCATTCGCAGCAACCAAT |
| ChiA-R | AACTGCAGCGGTAAGCTGTGAACCTT |
| ChiB-F | GGAATTCGCAGTGGCTGCACTTAG |
| ChiB-R | TGCTCTAGACTGCTTTTCGTTGCCGAA |
| ChiC-F | GGAATTCATGAACCCTATAGCTAAACT |
| ChiC-R | TGCTCTAGAGCCATTATCAATCGCTCTAA |
| HexA-F | CGGGATCCATGAAACTAAGATTATTACC |
| HexA-R | TGCTCTAGATTTAACGATTATTTCATCTAA |
| HexB-F | CGGGATCCATGGCGTTATTTAGCAAGTAT |
| HexB-R | TGCTCTAGACTCAACAGGTAACGCACG |
| CdxA-F | CGCGGATCCGATGAAAAATAAGCACTGC |
| CdxA-R | AGGCGCGCCAACGTACATACCAAGTCCC |
| NagA-F | GCTCTAGACCCACACCTAAACAAGGT |
| NagA-R | ACCGTGATATCGCTAGTAGCTAGCAGCTA |
a
Forward (-F) and reverse (-R) primers are indicated.
b
Restriction sites are underlined.