Figure 4.

Immunoprecipitation of a human melanoma protein by immunoconjugate G71–1. The human melanoma line A2058 was equilibrated with the immunoconjugate and lysed with detergent, and the immunoconjugate-protein complex was isolated on protein-G beads and analyzed by PAGE using a 4–15% gradient gel. Lane M, Protein molecular weight markers. Lane 1, immunoconjugate alone. Lane 2, immunoconjugate with associated melanoma protein. The same experiment was done with immunoconjugate E26–1, which also immunoprecipitated a single protein with a molecular mass of 250 kDa.