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. 2000 Feb 1;97(3):1119–1124. doi: 10.1073/pnas.97.3.1119

Figure 2.

Figure 2

Effect of β-arrestin and dynamin mutants on agonist-mediated internalization of G protein-coupled receptors. Sensitivity to β-arrestin 1 (βarr) V53D, β-arrestin 1 S412D, and dynamin (dyn) K44A is shown after a 30-min agonist stimulation. (a) Effect of dominant-negative mutants on internalization of Gs-coupled receptors. The Flag-β2AR and Flag-β1AR were stimulated by isoproterenol (10 μM), the Flag-VIP1R was stimulated by VIP (0.1 μM), and the Flag-A2BR was stimulated by adenosine (1 μM) for 30 min. (b) The Gi-coupled HA-μOR and HA-M2 MR were stimulated with etorphine (500 nM) or acetylcholine (ACh) (100 μM) for 30 min. (c) Effect of dominant-negative mutants on internalization of Gq-coupled receptors. The Flag-ETBR was stimulated with endothelin-1 (0.1 μM), the HA-AT1AR was stimulated with angiotensin II (0.1 μM), and the HA-M2 MR was stimulated with ACh (100 μM). Data represent the means ± SEM of 3–5 independent experiments. *, P < 0.01; **, P < 0.001.