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Effect of MDC treatment on internalization of the model G protein-coupled receptors. HEK 293 cells transiently transfected with Flag-β2AR, Flag-ETBR, or HA-AT1AR were treated with MDC (400 μM) for 30 min before stimulation with isoproterenol (10 μM), endothelin-1 (0.1 μM), or angiotensin II (1 μM), respectively. Data are mean ± SEM of four experiments done in duplicate. **, P < 0.01.
