Figure 3.

Induction of epiregulin mRNA by AII, ET-1, and α-Thr. Poly(A)⁺ RNA samples were prepared from serum-starved RASM cells that were either untreated or stimulated with AII (1 μM), ET-1 (100 nM), or α-Thr (10 nM) for the indicated times. (A and B) RNA blots (3 μg per lane) were probed with random-primed ³²P-labeled cDNA containing rat epiregulin (A) or hGAPDH (B). Numbers on left are kb. (C) Epiregulin (4.8-kb transcript) expression was quantitated by phosphorimage analysis and normalized by comparison with hGAPDH levels (B). Results are representative of independent experiments performed on two separate occasions.