Figure 2.

(A) magellan3 insertions in the E. coli lamB gene. A library of magellan3 insertions in E. coli was constructed by conjugating a suicide vector encoding the mariner transposase and magellan3 into a recipient strain. Mutants that were resistant to lysis with a virulent λ phage were selected. To map those insertions that were within the lamB gene, individual colonies were selected and PCR was performed with a primer that hybridized within the lamB gene and a primer that hybridized to the inverted repeat of magellan3. (B) PCR products were analyzed on an agarose gel.