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mariner transposition system for mycobacteria. Plasmid pMycoMar, which encodes the Himar1 transposase and the magellan4 minitransposon, was introduced into M. smegmatis by electroporation. After overnight recovery at 30°C without selection, transformants were plated on medium containing kanamycin and incubated at either 30°C (to determine the total number of transformants) or 39°C (to isolate insertion mutants).
