Figure 5.

RNase protection analysis of OCTN2, E2, and E3 expression. (a) Total RNA from liver, heart, kidney, lung, brain, spleen, and skeletal muscle from a wild-type mouse hemizygous for YAC A94G6 was hybridized with a probe specific for both murine and human OCTN2. (b) Total RNA from liver and kidney of a del¹¹/del¹¹ mouse hemizygous for YAC A94G6 (YAC −/−) and of a wild-type mouse (wt) was hybridized with a 208-bp probe specific for E2. (c) Total RNA from liver and kidney of a del¹¹/del¹¹ mouse hemizygous for YAC A94G6 (YAC −/−) and of a wild-type mouse (wt) was hybridized with a 280-bp probe specific for E3. A probe for β-actin was used as a control.