Figure 6.

Neurodegeneration in NP-C mouse brain revealed by amino–cupric–silver staining. (A) Layers I–III of the cortex of a 22-day-old normal mouse showing absence of degenerating structures. (B) Section through the temporal neocortex (CX), corpus callosum (CC), cornu ammonis field CA1 (CA), and dentate gyrus (DG) of a 22-day-old NP-C mouse. Many degenerating terminals are present in the neuropil of the cerebral cortex (*), although there are few labeled cell bodies (c.f., C). (C) Section through the temporal neocortex, corpus callosum (CC), cornu ammonis field CA1 (CA), and dentate gyrus (DG) of a 72-day-old NP-C mouse. Large numbers of labeled cell bodies are present in the cerebral cortex (arrows) in addition to the degenerating terminals. Marked degeneration is also observed in the neuropil of the hippocampus, although few degenerating cell bodies are observed in the strata pyramidale (SP) and granulosum (SG). [Bars = 250 μm (A and B) and 330 μm (C).]