Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1981 Dec;44(4):737–744.

Collagen-induced arthritis in the rat: modification of immune and arthritic responses by free collagen and immune anti-collagen antiserum.

N A Staines, T Hardingham, M Smith, B Henderson
PMCID: PMC1554980  PMID: 7319558

Abstract

Inbred Wistar strain rats developed a polyarthritic disease when injected intradermally with Freund's incomplete adjuvant (FIA) containing porcine or bovine type II collagen (CII). In contrast, neither allogeneic rat CII nor porcine proteoglycan monomer were arthritogenic, although both were, to some extent, immunogenic. Animals passively transfused with immune serum containing anti-CII antibodies did not develop arthritis and showed greatly reduced anti-CII humoral immune responses (measured by an enzyme-linked immunosorbent assay) when the serum was administered at the time of and subsequent to challenge with arthritogenic porcine CII. Similarly, the intravenous injection of animals sequentially with 100 microgram soluble CII and 1 ml immune anti-CII antiserum, 9 and 8 days respectively before challenge with arthritogenic CII, abrogated the arthritic response and depressed the humoral anti-porcine CII and anti-rat CII antibody titres more than tenfold. It is concluded that the immune status of the recipient rats with respect to CII is crucially important in determining the nature of the immune and arthritic response to CII appropriately administered in FIA.

Full text

PDF
737

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Billingham M. E., Gordon A. H. The role of the acute phase reaction in inflammation. Agents Actions. 1976 Feb;6(1-3):195–200. doi: 10.1007/BF01972208. [DOI] [PubMed] [Google Scholar]
  2. Courtenay J. S., Dallman M. J., Dayan A. D., Martin A., Mosedale B. Immunisation against heterologous type II collagen induces arthritis in mice. Nature. 1980 Feb 14;283(5748):666–668. doi: 10.1038/283666a0. [DOI] [PubMed] [Google Scholar]
  3. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  4. Eyre D. R., Muir H. The distribution of different molecular species of collagen in fibrous, elastic and hyaline cartilages of the pig. Biochem J. 1975 Dec;151(3):595–602. doi: 10.1042/bj1510595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Glenn E. M., Gray J. Adjuvant-induced polyarthritis in rats: biologic and histologic background. Am J Vet Res. 1965 Sep;26(114):1180–1194. [PubMed] [Google Scholar]
  6. Gosslau B., Barrach H. J. Enzyme-linked immunosorbent microassay for quantification of specific antibodies to collagen type I, II, III. J Immunol Methods. 1979;29(1):71–77. doi: 10.1016/0022-1759(79)90127-3. [DOI] [PubMed] [Google Scholar]
  7. Hardingham T. E., Ewins R. J., Muir H. Cartilage proteoglycans. Structure and heterogeneity of the protein core and the effects of specific protein modifications on the binding to hyaluronate. Biochem J. 1976 Jul 1;157(1):127–143. doi: 10.1042/bj1570127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  9. Morgan K., Clague R. B., Shaw M. J., Holt P. J. Native type II collagen-induced arthritis in the rat. I. Incidence and humoral response to collagen. Ann Rheum Dis. 1980 Jun;39(3):285–290. doi: 10.1136/ard.39.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. STEGEMANN H. Mikrobestimmung von Hydroxyprolin mit Chloramin-T und p-Dimethylaminobenzaldehyd. Hoppe Seylers Z Physiol Chem. 1958;311(1-3):41–45. [PubMed] [Google Scholar]
  11. Stuart F. P., Scollard D. M., McKearn T. J., Fitch F. W. Cellular and humoral immunity after allogeneic renal transplantation in the rat. V. Appearance of anti-idiotypic antibody and its relationship to cellular immunity after treatment with donor spleen cells and alloantibody. Transplantation. 1976 Nov;22(5):455–466. doi: 10.1097/00007890-197611000-00008. [DOI] [PubMed] [Google Scholar]
  12. Stuart J. M., Cremer M. A., Dixit S. N., Kang A. H., Townes A. S. Collagen-induced arthritis in rats. Comparison of vitreous and cartilage-derived collagens. Arthritis Rheum. 1979 Apr;22(4):347–352. doi: 10.1002/art.1780220406. [DOI] [PubMed] [Google Scholar]
  13. Stuart J. M., Cremer M. A., Kang A. H., Townes A. S. Collagen-induced arthritis in rats. Evaluation of early immunologic events. Arthritis Rheum. 1979 Dec;22(12):1344–1351. doi: 10.1002/art.1780221205. [DOI] [PubMed] [Google Scholar]
  14. Trentham D. E., Dynesius R. A., David J. R. Passive transfer by cells of type II collagen-induced arthritis in rats. J Clin Invest. 1978 Aug;62(2):359–366. doi: 10.1172/JCI109136. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Trentham D. E., McCune W. J., Susman P., David J. R. Autoimmunity to collagen in adjuvant arthritis of rats. J Clin Invest. 1980 Nov;66(5):1109–1117. doi: 10.1172/JCI109940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Trentham D. E., Townes A. S., Kang A. H. Autoimmunity to type II collagen an experimental model of arthritis. J Exp Med. 1977 Sep 1;146(3):857–868. doi: 10.1084/jem.146.3.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Trentham D. E., Townes A. S., Kang A. H., David J. R. Humoral and cellular sensitivity to collagen in type II collagen-induced arthritis in rats. J Clin Invest. 1978 Jan;61(1):89–96. doi: 10.1172/JCI108929. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES