Table 2.
Genotype | Time of harvest, days | Condition of embryo | No. of embryos (% total) |
---|---|---|---|
+/+ | 8.5 | Healthy | 9 (26) |
+/− | 8.5 | Healthy | 19 (54) |
−/− | 6.5 | Degenerating | 5 (15) |
No PCR result | 6.5 | Degenerating | 2 (5) |
Total no. of embryos | 35 (100) |
Embryos were flushed from the uterus at day 3.5. Healthy embryos were harvested at day 8.5 (i.e., 5 days in culture), while degenerating embryos were harvested at day 6.5 (i.e., 3.5 days in culture) for nested PCR analysis.