Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1982 Jun;46(2):343–351.

Stimulation of monocyte production by an endogenous mediator induced by a component from Listeria monocytogenes.

D T Shum, S B Galsworthy
PMCID: PMC1555393  PMID: 6806176

Abstract

A monocytosis-producing activity (MPA) is present in a saline-extractable material (SE) from Listeria monocytogenes. The mechanism of stimulation of monocyte production by SE was studied. Serum obtained from mice at appropriate times after injection of SE induced monocytosis in normal recipients. The monocytosis-inducing activity present in serum differed from SE with respect to timing of the monocytosis, fractionation pattern on a Sephadex G-200 column, and thermolability. The minimum dose of SE capable of producing a monocytosis was 100 micrograms. Antibody to SE capable of detecting SE at a concentration of greater than 5 micrograms/ml failed to detect SE in samples of active serum. Therefore it seemed highly unlikely that activity in serum was due to the presence of trace amounts of SE. The activity present in serum was thermolabile and had a molecular weight of about 38,000. The data are consistent with the hypothesis that injection of SE caused the production or release of an endogenous mediator capable of stimulating monocytosis.

Full text

PDF
343

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buhles W. C., Jr, Shifrine M. Increased bone marrow production of granulocytes and mononuclear phagocytes induced by mycobacterial adjuvants: improved recovery of leukopoiesis in mice after cyclophosphamide treatment. Infect Immun. 1978 Apr;20(1):58–65. doi: 10.1128/iai.20.1.58-65.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cheers C., McKenzie I. F. Resistance and susceptibility of mice to bacterial infection: genetics of listeriosis. Infect Immun. 1978 Mar;19(3):755–762. doi: 10.1128/iai.19.3.755-762.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eaves A. C., Bruce W. R. In vitro production of colony-stimulating activity. I. Exposure of mouse peritoneal cells to endotoxin. Cell Tissue Kinet. 1974 Jan;7(1):19–30. doi: 10.1111/j.1365-2184.1974.tb00395.x. [DOI] [PubMed] [Google Scholar]
  5. Galsworthy S. B., Gurofsky S. M., Murray R. G. Purification of a monocytosis-producing activity from Listeria monocytogenes. Infect Immun. 1977 Feb;15(2):500–509. doi: 10.1128/iai.15.2.500-509.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goud T. J., Schotte C., van Furth R. Identification and characterization of the monoblast in mononuclear phagocyte colonies grown in vitro. J Exp Med. 1975 Nov 1;142(5):1180–1199. doi: 10.1084/jem.142.5.1180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Klimpel G. R., Henney C. S. BCG-induced suppressor cells. I. Demonstration of a macrophage-like suppressor cell that inhibits cytotoxic T cell generation in vitro. J Immunol. 1978 Feb;120(2):563–569. [PubMed] [Google Scholar]
  8. McGregor D. D., Logie P. S. The mediator of cellular immunity. VI. Effect of the antimitotic drug vinblastine on the mediator of cellular resistance to infection. J Exp Med. 1973 Mar 1;137(3):660–674. doi: 10.1084/jem.137.3.660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Metcalf D. Acute antigen-induced elevation of serum colony stimulating factor (CFS) levels. Immunology. 1971 Sep;21(3):427–436. [PMC free article] [PubMed] [Google Scholar]
  10. Mitsuyama M., Takeya K., Nomoto K., Shimotori S. Three phases of phagocyte contribution to resistance against Listeria monocytogenes. J Gen Microbiol. 1978 May;106(1):165–171. doi: 10.1099/00221287-106-1-165. [DOI] [PubMed] [Google Scholar]
  11. North R. J. Cellular mediators of anti-Listeria immunity as an enlarged population of short lived, replicating T cells. Kinetics of their production. J Exp Med. 1973 Aug 1;138(2):342–355. doi: 10.1084/jem.138.2.342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. North R. J. Importance of thymus-derived lymphocytes in cell-mediated immunity to infection. Cell Immunol. 1973 Apr;7(1):166–176. doi: 10.1016/0008-8749(73)90193-7. [DOI] [PubMed] [Google Scholar]
  13. STANLEY N. F. Studies on Listeria monocytogenes; isolation of a monocytosis-producing agent. Aust J Exp Biol Med Sci. 1949 Mar;27(2):123–131. doi: 10.1038/icb.1949.12. [DOI] [PubMed] [Google Scholar]
  14. Scher M. G., Beller D. I., Unanue E. R. Demonstration of a soluble mediator that induces exudates rich in Ia-positive macrophages. J Exp Med. 1980 Dec 1;152(6):1684–1698. doi: 10.1084/jem.152.6.1684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shum D. T., Galsworthy S. B. Stimulation of monocyte precursors in vivo by an extract from Listeria monocytogenes. Can J Microbiol. 1979 Jun;25(6):698–705. doi: 10.1139/m79-101. [DOI] [PubMed] [Google Scholar]
  16. Skamene E., Kongshavn P. A. Phenotypic expression of genetically controlled host resistance to Listeria monocytogenes. Infect Immun. 1979 Jul;25(1):345–351. doi: 10.1128/iai.25.1.345-351.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Skamene E., Kongshavn P. A., Sachs D. H. Resistance to Listeria monocytogenes in mice: genetic control by genes that are not linked to the H-2 complex. J Infect Dis. 1979 Feb;139(2):228–231. doi: 10.1093/infdis/139.2.228. [DOI] [PubMed] [Google Scholar]
  18. Stanley E. R., Cifone M., Heard P. M., Defendi V. Factors regulating macrophage production and growth: identity of colony-stimulating factor and macrophage growth factor. J Exp Med. 1976 Mar 1;143(3):631–647. doi: 10.1084/jem.143.3.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stanley E. R., Metcalf D. The molecular weight of colony-stimulating factor (CSF). Proc Soc Exp Biol Med. 1971 Jul;137(3):1029–1031. doi: 10.3181/00379727-137-35721. [DOI] [PubMed] [Google Scholar]
  20. Tadayon R. A., Carroll K. K., Murray R. G. Purification and properties of biologically active factors in lipid extracts of Listeria monocytogenes. Can J Microbiol. 1970 Jun;16(6):535–544. doi: 10.1139/m70-090. [DOI] [PubMed] [Google Scholar]
  21. Van Furth R., Diesselhoff-den Dulk M. C., Mattie H. Quantitative study on the production and kinetics of mononuclear phagocytes during an acute inflammatory reaction. J Exp Med. 1973 Dec 1;138(6):1314–1330. doi: 10.1084/jem.138.6.1314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Volkman A. The origin and fate of the monocyte. Ser Haematol. 1970;3(2):62–92. [PubMed] [Google Scholar]
  23. WISH L., FURTH J., STOREY R. H. Direct determinations of plasma, cell, and organ-blood volumes in normal and hypervolemic mice. Proc Soc Exp Biol Med. 1950 Jul;74(3):644–648. doi: 10.3181/00379727-74-18003. [DOI] [PubMed] [Google Scholar]
  24. Willoughby D. A., Coote E., Spector W. G. A monocytogenic humoral factor released after lymph node stimulation. Immunology. 1967 Feb;12(2):165–178. [PMC free article] [PubMed] [Google Scholar]
  25. Zinkernagel R. M., Althage A., Adler B., Blanden R. V., Davidson W. F., Kees U., Dunlop M. B., Shreffler D. C. H-2 restriction of cell-mediated immunity to an intracellular bacterium: effector T cells are specific for Listeria antigen in association with H-21 region-coded self-markers. J Exp Med. 1977 May 1;145(5):1353–1367. doi: 10.1084/jem.145.5.1353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. van Furth R., Cohn Z. A. The origin and kinetics of mononuclear phagocytes. J Exp Med. 1968 Sep 1;128(3):415–435. doi: 10.1084/jem.128.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. van Furth R., Diesselhoff-Den Dulk M. M. The kinetics of promonocytes and monocytes in the bone marrow. J Exp Med. 1970 Oct 1;132(4):813–828. doi: 10.1084/jem.132.4.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. van Waarde D., Hulsing-Hesselink E., Sandkuyl L. A., van Furth R. Humoral regulation of monocytopoiesis during the early phase of an inflammatory reaction caused by particulate substances. Blood. 1977 Jul;50(1):141–154. [PubMed] [Google Scholar]
  29. van Waarde D., Hulsing-Hesselink E., van Furth R. A serum facted by newborn calf serum. Cell Tissue Kinet. 1976 Jan;9(1):51–63. [PubMed] [Google Scholar]
  30. van Waarde D., Hulsing-Hesselink E., van Furth R. Properties of a factor increasing monocytopoiesis (FIM) occurring in serum during the early phase of an inflammatory reaction. Blood. 1977 Oct;50(4):727–742. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES