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rad3Δ is more TBZ sensitive than rad3.a. Cultures were grown at 30°C in liquid, complete media to 1 × 106 cells/ml. Two-fold serial dilutions of cultures were made onto complete agar media (YE5S) and complete agar media containing 20 μg/ml TBZ (20 μg/ml) and incubated at 30°C. Pictures of YE5S plates were taken after two days of growth, while those of YE5S + 20 μg/ml TBZ plates were taken after four days. Wildtype (TE696), rad26Δ (TE257), rad3Δ (TE890) and rad3.a (TE571).
