Figure 6.

Rad26-GFP spots form in Bleomycin-treated rad26-GFP cells, but not in G2/M arrested cdc25.22 rad26-GFP cells. Cultures were grown at 30°C in liquid, complete media to O.D. 0.3 and then treated with 5 mU/ml of Bleomycin for 3 hours. Cells were then prepared for microscopy following the Triton X-100 extraction method (see Methods). This experiment was repeated twice with similar results, one of which is shown. The procedure used to arrest the cdc25.22 rad26-GFP culture before mitosis is outlined in Methods. rad26-GFP (TE1197), cdc25.22 rad26-GFP (TE1198)