Figure 7.

Rad26-GFP spots form in Bleomycin-treated checkpoint radΔ cells. Cultures were grown at 30°C in liquid, complete media to O.D. 0.3 and then treated with 5 mU/ml of Bleomycin for 3 hours. Cells were then prepared for microscopy following the Triton X-100 extraction method (see Methods). This experiment was repeated three times with similar results, one of which is shown. rad26-GFP (TE1197), hus1Δ rad26-GFP (TE1192) rad1Δ rad26-GFP (TE1194), rad9Δ rad26-GFP (TE1193) and rad17Δ rad26-GFP (TE1196)