Figure 5.

Induction of apoptosis by HIV-1 envelope proteins. Annexin V staining and TUNEL assay of PBMCs. Annexin V and propidium iodide staining of PBMCs treated with HIV-1 JRFL envelope for 16 h is shown in A. The percentage of Annexin V-positive/propidium iodide-negative cells is indicated in the lower right quadrant of the histograms in A. TUNEL assay of PBMCs untreated or treated with anti-Fas mAb CH11 or HIV-1 JRFL envelope for 56 h is shown in B. mcf is displayed on the x axis and total cell counts on the y axis. The percentage of TUNEL-positive cells is presented for each panel.