Figure 5.

In situ hybridization analysis of Hsc73 in the brains of ovx rats treated with EB and P. The brains from ovx rats treated with sesame oil vehicle, EB and EB + P (see Materials and Methods) were dissected and sectioned as in Fig. 2. In situ hybridization and quantitative analysis also were performed as in Fig. 2. Each bar represents the mean ± SEM of two animals per group. ∗ indicates a significant difference (P < 0.05) between vehicle- and hormone-treated animals. ∗ above the cVMH bars indicates a significant difference (P < 0.05) between animals treated with EB only and those that received EB + P.