Figure 2.

Xenopus, mouse, and human Gab1 proteins are structurally conserved. The putative full-length Gab1 cDNA was cloned by PCR from a Xenopus oocyte cDNA library. The alignment of XGab1 amino acid sequences with those of the human and mouse Gab1 proteins is shown (Holgado-Madruga et al., 1996; Weidner et al., 1996). Conserved residues are highlighted in gray; the PH domain and MBD are denoted by a full or dashed underline, respectively. Lines above the sequences indicate the conserved PI3K, Crk, PLCγ, RasGAP, or SHP-2 binding sites. Potential ERK phosphorylation sites are denoted by lines below the sequences. The Grb2 carboxy-terminal SH3 domain binding sites and the MBM are marked by underbracket and a dotted underline, respectively.