Figure 8.

The MBD of Gab1 represses FGFR1-mediated cell cycle, but not GVBD induced by progesterone. (A) Oocytes injected with FGFR1 and FRS2 mRNAs (4 ng each/oocyte) in the presence or absence of wt or mutant of MBD of Gab1 mRNAs (14 ng/oocyte) were stimulated with 200 ng/ml human recombinant basic FGF. (B) Oocytes left uninjected or injected with wt or mutant MBD mRNAs (14 ng/oocyte) were stimulated with 5 μM progesterone. Histograms represent the number of oocytes with visible GVBD over the number of oocytes injected and shown as percentage of GVBD. The data are representative of three independent experiments. Lysates prepared from oocytes were analyzed by immunoblot conducted with specific antibodies raised against HA, to detect HA-tagged MBD, phospho-MAPK, MAPK, phospho-JNK, or JNK1/2.