Figure 2.

PTEN nuclear accumulation is facilitated by mutations targeting the 370–385 region of the PTEN C-terminal tail. U87MG cells were transfected with wild-type (wt) PTEN, PTEN mutations targeting the CK2 phosphorylation sites (DMA [S370A/S385A], TMA [S380A/T382A/T383A], QMA [S370A/S380A/T382A/T383A/S385A], S370A, S380A, T382A, T383A, and S385A mutations), or PTEN mutations targeting the caspase-3 cleavage sites (D384N, CM2 [D371N/D375N], and CM3 [D371N/D375N/D384N] mutations), and cells were processed for immunofluorescence as in Figure 1. (A–C) Representative images of the localization of some recombinant PTEN proteins are shown. Quantitation of the subcellular localization of the indicated PTEN proteins, using the anti-PTEN 425A mAb (B), or the anti-PTEN 421B mAb or an anti-PTEN N-terminal polyclonal antibody (C). Data are presented as in Figure 1B.