Figure 4.

The nuclear accumulation of PTEN is affected in mutations that compromise catalytic activities. U87MG cells were transfected with wild-type (wt) PTEN, QMA (S370A/S380A/T382A/T383A/S385A), 1–375, or the catalytically inactive mutants (C124S and G129E) alone or in combination with the QMA (QMA/C124S and QMA/G129E) or the 1–375 (1–375/C124S and 1–375/G129E) mutations, and cells were processed for immunofluorescence as in Figure 1. (A) Representative images of the localization of the indicated PTEN proteins are shown (FITC-immunoglobulin). Nuclei were costained with Hoechst 33258. (B) Quantitation of the subcellular localization of the indicated PTEN proteins. Data are presented as in Figure 1B.