Figure 5.

The mitochondrial precursors for Mdj1p and Hsp60p accumulate upon shifting mmp37Δ cells to the nonpermissive temperature of 37°C. (A) Strains K699 (WT) and YLM2814 (mmp37Δ) were grown in YEP 2% galactose medium at 30°C until the cells reached midlog phase. Subsequently at time point zero, the cells for each strain were diluted to an OD₆₀₀ of 0.125 in YEP 2% galactose medium, and each strain was divided into two cultures. One culture was maintained at 30°C, whereas the second culture was shifted to 37°C, and the OD₆₀₀ for each culture was determined at 2, 4, 6, 8, 10, 12, and 25 h. (B) At time points 0, 4, 8, and 12 h an aliquot of cells was removed from each culture, and mitochondrial lysates were prepared. Ten micrograms of each mitochondrial lysate was separated by SDS-PAGE and analyzed by Western blotting with antisera specific to each of the indicated proteins. The asterisk (*) indicate the position of the Mdj1p and Hsp60p precursors.