Figure 7.

Import inhibition observed in mmp37Δ mitochondria can be overcome by unfolding the preprotein before import. (A–C) The import of radiolabeled pSu9(1-69)DHFR preproteins into wild-type and mmp37Δ mitochondria was performed as described in Figure 6. (A) Import of pSu9(1-69)DHFR bearing a wild-type folded DHFR moiety. (B) Import of a pSu9(1-69)DHFR^MUT, a derivative bearing an folding incompetent DHFR moiety. (C) Import of pSu9(1-69)DHFR, which had been chemically denatured in 8 M urea before dilution into the import assay. After import, samples were further processed as described above in Figure 6.