Figure 2.

BFA/H89 treatment produces ER localization of Golgi membrane proteins with a more granular redistribution of GM130. Wild-type HeLa cells were treated with BFA supplemented with H89, and then cells were fixed and costained for Sec61p (B) with GalT (A), Giantin (C), or GM130 (D) each individually. GalT and Giantin redistributed to an ER and showed extensive colocalization with Sec61p, whereas GM130 displayed more granular ER-like pattern: beads-on-a-string (arrows). All micrographs were taken with a CARV II spinning disk confocal device. Bar, 10 μm.