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. 2006 Sep;17(9):4118–4129. doi: 10.1091/mbc.E06-02-0101

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© 2006 by The American Society for Cell Biology

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Figure 6. — A cleavage of ErbB4 is required for differentiation of HC11 cells. (A) Pools of HC11 cells stably transfected with pLXSN-HER4, -HER4^KD, or -HER4^VA were cultured overnight in serum-free media and treated for 30 min with HB-EGF. Membrane and cytoplasmic extracts were fractionated and analyzed by immunoblot (IB) using an ErbB4 mAb (Neomarkers). Molecular weights are shown at left. n.s., nonspecific band. (B) Western analysis to detect tyrosine phosphorylation of ErbB1, -2, -3, and -4 IPs from serum-starved HC11-HER4^VA cells treated with EGF (ErbB1) or HRG (ErbB2–4) for 10 min. (C) Serum-starved cells cultured 30 min ± HB-EGF. Arrows indicate nuclear localization. DAPI staining of nuclei is shown in bottom panels. Indirect immunofluorescence to detect ErbB4 is shown in the top panels.