Figure 3.

Western blotting of purified IL-18BP isoforms. COS supernatants purified over Talon columns were subjected to 10% SDS/PAGE under reducing (50 mM DTT) conditions and transferred to nitrocellulose. (A) Blot was probed with mAb anti-his6-tag. (B) Blot was probed with rabbit anti-human IL-18BPa. Molecular weights are indicated on the vertical axis. Mock represents supernatants from COS cells transfected with the empty pEF-BOS vector and subjected to the same purification as the IL-18BP isoforms. CHO-derived IL-18BPa is shown on the right and the lane numbers indicate IL-18BPa in ng.