Figure 1.

Patch-clamp recording of PCs. Spontaneous inward currents elicited GABAergic afferents in control conditions (A1). These currents are not present in a cell from a TTX-treated cerebellum (A2). (B) Several superimposed traces from the same cell by applying stimuli of increasing intensity (from 1 V to 100 V); no evoked current is present. (C) In the same cell, the application of 50 μM kainate induces a large inward current, suggesting that glutamate receptors are functional. Holding potential, −70 mV. (Inset) Schematic representation of a PC with a CF impinging on the rare spines of proximal dendrites, and many parallel fibers (PF) ending on spiny branchlets with numerous spines.