Figure 2.

Effect of testosterone and other steroids on secretion of sβAPPα and Aβ from murine N2a neuroblastoma cells transfected with human βAPP695. (a) Representative autoradiographic analysis of sβAPPα (Lower) and Aβ (Upper). (b) Effects of steroids on the release of sβAPPα (Left) and Aβ (Right) represented as means ± SD, n = 3 (statistically different from controls by Student's t test; *, P < 0.01; **, P < 0.02). Effects of 17β-estradiol and testosterone were not statistically different from each other at either 200 nM or 1 μM. (c) Quantitation of Aβ release in response to treatment with 200 nM testosterone for 0–14 days. Data are represented as means ± SD, n = 3 (statistically different from control cells incubated for the same duration in the absence of hormone; *, P < 0.02).