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. 2003 Jun;71(6):3000–3009. doi: 10.1128/IAI.71.6.3000-3009.2003

FIG. 2.

FIG. 2.

Presence of active mammalian TGF-β1, as well as activation of latent TGF-β1 in the presence of NO, in A. stephensi midguts. (A) Mosquito cell proteins did not cross-react with anti-TGF-β1 used in the Quantikine ELISA. In the left panel, 22 μg of mosquito cell proteins (lane A) and 10 ng of human TGF-β1 (lane B) are shown after polyacrylamide gel electrophoresis and Coomassie brilliant blue staining. Identical protein samples transferred to nitrocellulose (right panel) were incubated with anti-human TGF-β1 as described in the text. Note the strong detection of human TGF-β1 in lane B (arrow) and the lack of cross-reacting mosquito proteins in lane A. (B) Lysates of mosquito midguts (50 per lysate) were prepared at various times after blood feeding (“incubation time”) indicated on the x axis. The lysates were treated as described in the boxed legend and in Materials and Methods. Active TGF-β1 was quantified in the samples by using the Quantikine ELISA.

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