FIG. 2.

CLUSTAL X alignment of C. posadasii Gel1p homologs. The sequences of reported β-1,3-glucanosyltransferases of A. fumigatus (AfGel1p), N. crassa (NcGel1p), and S. cerevisiae (ScGas1p) were compared to the translated, full-length ORF of the GEL1 gene of C. posadasii (CpGel1p). Conserved catalytic motifs are boxed (solid line), and the six aligned cysteine residues are indicated by enlarged, bold type. Predicted GPI anchor sites in the C-terminal region of the homologs are boxed (dotted line). The predicted signal peptide cleavage site of the CpGel1p is indicated between S¹⁸ and A¹⁹. The underlined residues of CpGel1p represent the translated sequence of the 495-bp gene fragment originally identified in the C. posadasii genomic database. GenBank accession numbers are provided in Table 1.