Figure 1.
Stable, high-level GFP-expressing B16F0 murine melanoma transductants in vitro. The murine malignant melanoma cell line B16F0 was transduced previously with the RetroXpress vector pLEIN, which expresses EGFP (19) and the neomycin resistance gene on the same bicistronic message (18). Stable, high-expression clones were selected in 800 μg/ml G418 (18). (Bar = 40 μm.)