Figure 2.
External images of murine melanoma (B16F0-GFP) metastasis in brain. Murine melanoma metastases in the mouse brain were imaged by GFP expression under fluorescence microscopy. Clear images of metastatic lesions in the brain can be visualized through the scalp and skull. See Materials and Methods for imaging equipment and procedures. (A) External GFP image of brain metastasis through the scalp and scull of an intact mouse 3 weeks after injection of 106 B16F0-GFP cells in the tail vein. (Bar = 1,280 μm.) (B) GFP image of same area as in A, with skull opened. (Bar = 1,280 μm.) (C) External image obtained of the tumor in the brain of the nude mouse on day 14 after GFP tumor cell injection. (Bar = 1,280 μm.) (D) Same as C, day 19 after injection. (Bar = 1,280 μm.) (E) Same as C and D, day 25 after injection. (Bar = 1,280 μm.) (F) Brain tumor growth curve determined by external images (C–E).