TABLE 2.

Characteristics of ORFs and deduced amino acid sequences present in the sequenced DNA fragment

ORF	Gene	Product size (no. of amino acids)	ORF location (nt)a	Protein (description) to which ORF product exhibits homology	Source	Identityb		Accession no.
						%	Range (aa)
1	tnpA	136	3-413	TnpA; transposase of Tn1721, truncated	E. coli	95	135	P51565
2	ycjA	107	791-468	YcjA; putative bacterial repressor protein	Plasmid R64; S. enterica serovar Typhimurium	100	107	BAB91595
3	ydaA	377	896-2029	YdaA; putative permease	Plasmid R64; S. enterica serovar Typhimurium	90	377	BAB91596
4	ydbA	205	2939-2322	YdbA	Plasmid R64; S. enterica serovar Typhimurium	96	205	BAB91597
5	ibfA	518	4686-3130	lbfA; putative ABC transporter	Plasmid R64; S. enterica serovar Typhimurium	95	518	BAB91598
6	ydeA	196	5539-4949	YdeA	Plasmid R64; S. enterica serovar Typhimurium	100	196	BAB91599
7	ydfA	85	5796-5539	YdfA; putative ABC transporter	Plasmid R64; S. enterica serovar Typhimurium	100	85	BAB91600
8	ydfB	712	6150-8288	YdfB; putative ABC transporter	Plasmid R64; S. enterica serovar Typhimurium	100	712	BAB91601
9	mck	133	8851-8450	Mck; involved in coordination of plasmid replication with cell division	Plasmid R64; S. enterica serovar Typhimurium	99	133	BAB91602
10	kor	76	9093-8863	Kor; involved in coordination of plasmid replication with cell division	Plasmid R64; S. enterica serovar Typhimurium	100	76	BAB91603
11	ydiA	96	9389-9679	YdiA; 93% identical to protein YebA on E. coli plasmid F	Plasmid R64; S. enterica serovar Typhimurium	100	96	BAB91604
12	ydiA	299	9669-10568	YdjA; 100% identical to protein YebB on E. coli plasmid F	Plasmid R64; S. enterica serovar Typhimurium	100	299	BAB91605
13	pifA*	443	11949-10618	Truncated ORF; homologous to residues 299 to 714 of 741 residues of protein PifA; phage T7 exclusion protein	Plasmid R64; S. enterica serovar Typhimurium	100	443	BAB91606
								P96329
14	tnpA	224	12299-12973	TnpA; transposase of IS903	Insertion sequence IS903	99	223	AAF30382
15	iroN	725	13385-15562	IroN; enterochelin and dihydrobenzoic acid receptor	PAI III of E. coli strain 536	99	725	CAC43424
16	iroE	318	16563-15607	IroE	PAI III of E. coli strain 536	99	318	CAC43425
17	iroD	409	17877-16648	IroD; putative ferric enterochelin esterase	PAI III of E. coli strain 536	99	409	CAC43426
18	iroC	1,261	21766-17981	IroC; putative ABC transporter	PAI III of E. coli strain 536	99	1,261	CAC43427.2
19	iroB	371	22895-21780	IroB; putative UDP-glucoronosyl and UDP-glucosyl transferase	PAI III of E. coli strain 536	100	371	CAD66179.1
20		89	23363-23094	Hypothetical protein	No significant homology
21		173	24692-24171	Hypothetical protein; putative integrase	E. coli strain K5	59	100	CAA54707
22		96	25164-24874	Hypothetical protein	E. coli strain K5	59	48	CAA54710
23		97	26026-25733	Hypothetical protein	E. coli	80	76	AAB40752
24	iss	102	26347-26039	Iss (increased serum survival and complement resistance)	E. coli strain 102	100	102	AAD41540
25		80	26948-26706	Hypothetical protein	E. coli strain EDL933	100	26	AE005326
26		112	26959-27297	Putative transposase of insertion element IS2	E. coli	98	109	P51026
27		263	27404-28195	Putative integrase of insertion element IS2	E. coli	97	262	P51026
28		54	28530-28366	Hypothetical protein; homologous to gene trp1328 on IS1328	Yersinia enterocolitica	63	50	CAB46575
29		397	29418-30611	Hypothetical protein; putative cobalamin synthesis protein	E. coli strain CFT073	65	397	NP_753181
30		103	30750-31061	Hypothetical protein; putative integrase	Shigella flexneri 2a strain 301	59	82	NP_709264
31		207	31248-31869	Truncated ORF; homologous to gene HCM1.201 on plasmid pHCM1; putative transposase	S. enterica serovar Typhi strain CT18	95	207	NP_569402

^aNucleotide positions from the start to stop codons in the pJS332 sequence.

^bIdentity is presented as percentage of amino acid identity between the pJS332 ORF and the best hit as determined with BLAST and FASTA. The range is the number of amino acids (aa) over which this identity exists.