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. 2003 Jun;71(6):3294–3301. doi: 10.1128/IAI.71.6.3294-3301.2003

FIG. 6.

FIG. 6.

TcdB1-500 inhibition of TcsL CPEs. HeLa cells were treated with TcdB1-500 plus PA for 30 min prior to treatment with TcsL. To enhance TcsL cytopathic activity, cells were treated with the toxin by using an acid pulse where cells were subjected to TcsL in acid medium (pH 4.0) for 10 min followed by replacement with neutral medium (pH 7.4) and TcdB1-500 plus PA. The cells were amended with inhibitor every 30 min for 12 h and then monitored for 18 h to determine CPEs. Samples and corresponding lines are labeled within the figure.