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Antibody response to the 27-kDa antigen in vaccinated mice. BALB/c mice were immunized subcutaneously with the 27-kDa antigen, Ribi-27-kDa, DDA-27-kDa, Ribi, DDA, and saline three times 2 weeks apart. Sera were collected and analyzed by ELISA for the presence of anti-27-kDa IgG1 and IgG2a antibodies. Each point represents the average and standard deviation of three independent experiments; in each experiment five individual mice were tested.
