Figure 2.

ZnT3 is expressed in the central nervous system. (A) ZnT3 mRNA levels in developing mouse brain, assessed by solution hybridization. Data are presented as mean ± SEM. (B–F) Expression of nlacZ (under the control of the ZnT3 promoter) in the central nervous system of ZnT3^+/− mice, as detected by 5-bromo-4-chloro-3-indolyl-β d-galactoside (X-gal) staining (white nuclei, indicated by black arrows). nlacZ is expressed in s granulosum of the dentate gyrus (B), the pyriform cortex (C), and the cochlear nucleus (D). nlacZ expression was not completely penetrant, as evident by the absence of staining (white arrows) in many areas where ZnT3 is normally expressed. (E and F) nlacZ is expressed in laminae I, II, III, and IV of the spinal cord dorsal horn. Sections were photographed under dark-field illumination. [Bars = 500 μm (B and D); 200 μm (C and E); 100 μm (F).]