TSQ fluorescence in the hippocampus.
(A) Computer-generated images of TSQ fluorescence in the
hippocampi of ZnT3+/+ (Left),
ZnT3+/− (Center), and
ZnT3−/− (Right) mice. The
bright fluorescence in the hilus (Hi), s oriens and s lucidum of CA3,
and s oriens and s radiatum of CA1 was reduced in the
ZnT3+/− hippocampus and undetectable in the
ZnT3−/− hippocampus. TSQ staining was also
reduced in the neocortex (N). TSQ fluorescence in the hippocampus of
the mutants was less than the autofluorescence of the overlying corpus
callosum (cc). (B) Quantification of TSQ fluorescence in
regions within the hippocampus by computer-assisted laser cytometry.
Data are expressed as mean ± SEM (n = 5).
(C) TSQ-stained section of a single seminiferous tubule from
the testis of a ZnT3−/− mouse. Spermatids
poised at the lumen of the tubule fluoresced with TSQ, similar to
wild-type spermatids (not shown). (D) TSQ-stained pancreas
from a ZnT3−/− mouse, illustrating a single
Islet of Langerhans, composed of beta cells that have an abundance of
histochemically reactive zinc packaged in secretory granules.
[Bars = 50 μm (C); 100 μm (D).]