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. 1999 Feb 16;96(4):1716–1721. doi: 10.1073/pnas.96.4.1716

Figure 6.

Figure 6

TSQ fluorescence in the hippocampus. (A) Computer-generated images of TSQ fluorescence in the hippocampi of ZnT3+/+ (Left), ZnT3+/− (Center), and ZnT3−/− (Right) mice. The bright fluorescence in the hilus (Hi), s oriens and s lucidum of CA3, and s oriens and s radiatum of CA1 was reduced in the ZnT3+/− hippocampus and undetectable in the ZnT3−/− hippocampus. TSQ staining was also reduced in the neocortex (N). TSQ fluorescence in the hippocampus of the mutants was less than the autofluorescence of the overlying corpus callosum (cc). (B) Quantification of TSQ fluorescence in regions within the hippocampus by computer-assisted laser cytometry. Data are expressed as mean ± SEM (n = 5). (C) TSQ-stained section of a single seminiferous tubule from the testis of a ZnT3−/− mouse. Spermatids poised at the lumen of the tubule fluoresced with TSQ, similar to wild-type spermatids (not shown). (D) TSQ-stained pancreas from a ZnT3−/− mouse, illustrating a single Islet of Langerhans, composed of beta cells that have an abundance of histochemically reactive zinc packaged in secretory granules. [Bars = 50 μm (C); 100 μm (D).]