FIG. 3.

IFN-γ up-regulates TLR4 and MD-2 mRNA in human intestinal epithelial cell lines. (A) Cells were cultured with IFN-γ (10 ng/ml) or medium for 6 h, and then total RNA was isolated. Northern blotting was performed as described in Materials and Methods, followed by hybridization with cDNA probes for TLR4 and GAPDH. (B) Cells were cultured with IFN-γ (10 ng/ml) or medium for 6 h, and then total RNA was isolated. MD-2 and GAPDH expression was assessed by RT-PCR using 2 μg of total RNA and primers.