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. 2003 Jun;71(6):3503–3511. doi: 10.1128/IAI.71.6.3503-3511.2003

FIG. 6.

FIG. 6.

Internalization of LPS by human intestinal epithelial cells and effects of IFN-γ on LPS uptake. (A) Cells were incubated for 0.5 (green line), 1 (red line), 2 (light-blue line), 3 (orange line), or 6 (blue line) h with 1 μg of Alexa Fluor 488-conjugated LPS/ml and then analyzed by FACS as described in Materials and Methods. (B) The change in mean fluorescence intensity (MFI) after Alexa Fluor 488-conjugated LPS incubation is shown. (C) The effects of IFN-γ (10 ng/ml) on LPS uptake, with results reported as means ± SD (error bars). *, P < 0.05 in comparison to respective control culture without IFN-γ. (D) Fluorescence microscopy image of Colo205 cells after 3 and 6 h of incubation with 1 μg of Alexa Fluor 488-conjugated LPS/ml.