TABLE 2.
Supernatanta | Transduction efficiency with the following recipients:
|
|||
---|---|---|---|---|
RV79 (TCP+)
|
Vc857 (TCP−)b
|
|||
Apr | Kmr | Apr | Kmr | |
N16961ΩK139 (glo::bla), tcp::Kmc | 5 × 10−1 | <1 × 10−6d | 5 × 10−4 | <1 × 10−6d |
O395ΩK139 (glo::bla), tcp::Kmc | 6 × 10−1 | <1 × 10−6d | 5 × 10−4 | <1 × 10−6d |
There was 106 PFU of phage K139 per ml in each supernatant.
A number of TCP− strains were tested as recipients.
Constructs with Kmr-oriR6K inserted in tcpI, aldA, tnp, and int were tested.
No Kmr colonies were found in 106 recipient cells.