FIG. 2.

Demonstration that a component of the anti-OspE Ab response elicited during infection in mice is paralog specific. E. coli harboring the full-length BBL39 or BBN38 construct was induced to express the r protein, and cell lysates were fractionated by SDS-PAGE and immunoblotted. Membranes were screened with sera collected from a mouse infected for 12 weeks with B. burgdorferi B31MIpc (A) or with infection sera preabsorbed with either BBL39 (B) or BBN38 (C) or both (D). All methods were as described in the text.