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Analysis of the BBL39 and BBN38 determinants necessary for binding of anti-OspE Abs elicited during infection in mice. Subfragments of BBL39 and BBN38 were generated as S-Tag fusion proteins, fractionated by SDS-PAGE, immunoblotted, and screened with anti-rOspE antisera or 12-week infection sera. All methods were as described in the text. Note that addition of the S-Tag to each OspE subfragment adds approximately 17 kDa to the molecular mass.
