FIG. 5.

Analysis of the binding of hfH to OspE and OspF proteins by use of an affinity ligand binding immunoblot format. r proteins were fractionated by SDS-PAGE and immunoblotted. As indicated, the blots were then incubated either with purified hfH or with blocking buffer prior to the addition of goat anti-fH antisera. All methods are described in the text. Asterisks indicate the migration position of FHL-1.