FIG. 6.

ELISA analysis of the abilities of recombinant BBL39 and BBN38 proteins or subfragments to bind polyclonal anti-OspE antisera and infection sera. Wells of ELISA plates were coated with OspE r proteins or subfragments. This and all other procedures are described in detail in the text. BBO39 served as a positive control for infection serum Ab binding, and BSA served as a negative control for both anti-rOspE antiserum and infection serum Ab binding. In one set of triplicate wells, anti-rOspE antisera were added (solid bars). Infection sera obtained from a mouse infected for 12 weeks with B. burgdorferi B31MIpc were added to another set of triplicate wells (open bars). Ab binding was measured as described in the text. Data are averages of three separate determinations. Error bars, standard deviations.