FIG. 7.

Analysis of the fH binding abilities of BBL39 and BBN38 subfragments. All methods were as described in the text. r proteins were immobilized in wells, and then one set of triplicate wells was incubated with a preparation of purified fH (solid bars) while a second set was incubated with blocking buffer (open bars). After a wash, either polyclonal anti-hfH antisera (A) or a monoclonal anti-hfH Ab (B) was added. Binding was measured as described in Materials and Methods and was expressed as the average of three determinations. Error bars, standard deviations.